Time-lapse seedling root camera system, designed by Kate Baldwin and Shih-Heng Su in the lab of Patrick Masson at the University of Wisconsin, Madison. We designed these boxes in 2017, and they are still being used heavily in the Masson Lab in 2022.

Supplies needed to make One Box for your own lab

• Supplies Purchase List
• PDF of ALL vector cut files to be used with a laser cutter to make the box (we used the laser cutter at Sector67) (or individual DXF files [in inches] for Black Acrylic, Clear Thick Acrylic, Clear Thin Acrylic, and White Acrylic)
• Script code to load on to Raspberry Pi for running the program.

Once you have all the acrylic pieces cut out, you can assemble them and hold it together with either clear packing tape, or acrylic welding glue like this. Use the part descriptions in the PDF to help figure out what parts go where.

Shih-Heng grows the seedlings on the plates held in the plate holders and then transfers them into the box set-up when they are ready. Contact Shih-Heng for more information on growing the seedlings.

Citation

The published paper that uses these boxes is: Cadaverine regulates biotin synthesis to modulate primary root growth in Arabidopsis by Gibbs et al.