Make sure your figures are good resolution, or vector based.
Please don’t use screen shots for real images.
Figures made in Power Point can look great in Word. Here is a good option for importing them as vector images. PPT isn’t my preferred tool as a professional, but it can work just fine if you import the images into Word with care.
If you are including a conceptual flow chart, or logic-diagram-type-of-image, don’t have everything pointing at everything.
We know all your subjects are connected, but when everything is pointing at everything, there is no real information. Have restraint.
Make the size of your canvas the real size it’s going to be in your proposal.
Proposals are always on 8.5X11 pages with usually 1 inch margins, so the largest your figure can be is 6.5inX9in. Working in real-life sizes is critical to maintaining reasonable font sizes.
Full-width images are great, and half-width images are great. But between 4 and 6 inches wide results in a narrow gutter for your body text.
It can work if you put the caption on the side, or if you are willing to have white space to the side. (But strict page limits always loom large!)
When mocking up the layout of your image, think about those outer boundaries/edges of your image. It’s probably going to be inserted into Word as a rectangle, so if you have one component way off to the side, it’s going to make the whole image larger, and space is always at a premium.
I like the look of a thin vertical figure in a proposal.
One possible downside is that PIs often want to recycle their images in Power Point, and a tall-thin figure isn’t so good for that.
Choose the minimum font size you are willing to use within the figure. This might be specified by your funding agency, but often it’s not.
8pt is a solid choice. I often see 4pt or smaller in grants (especially graphs), but I personally won’t go smaller than 6pt. Here is a menu showing what different options look like.
Using 12pt+ within a figure in a grant proposal is very unusual and looks weird.
Since you have chosen a minimum font size for your figure, use that size for almost all the text within the figure.
If you need to create a hierarchy of text, bold or underlining is a nice option. You can also use larger font size for the title, but often space is at a premium, and we have established that your minimum is perfectly readable.
For typeface, I generally stick to Arial. If you use the default font du jour in Microsoft, it’s a sign that you made it in power point. Not a big deal, but Arial is fairly timeless at this point and never looks out of place.
Contrast and color are tools. Use them judiciously. If you use high-contrast, jarring colors everywhere, then you’ve wasted one of your best tools.
It's like how if you write an entire email in all caps, then you can’t use capital letters to emphasize your MOST IMPORTANT POINT. Have restraint.
Instead of using random colors, do consider using a unified color theme to add to the professionalism of your figures.
There are many theories of color themes, but the easiest option is just to google, "color themes."
I also like to use Coolers, and the Color Guide built into Adobe Illustrator.
Use the same colors for each item throughout all the figures in your grantpaper.
Having the same item always be the same color will help your readers tremendously, and also make your project seem more professional and polished.
Generally, every color is either acceptable for black or white text, but not both.
If you are making a figure showing the effects of 3 treatments shown as 3 colors, use 3 colors of similar darkness so that you can use the same color of text (either white or black) on all three.
If your items are equally related to each other, then don't have two of the colors really similar and one outlier.
Red/Green deuteranopia is the most common form of color blindness, so never do a red/green heat map.
There are many other forms of color blindness too, so try to also use other indicators like labels and shapes to distinguish the items in your diagram.
Having items mis-colored in extreme ways and illicit the Stroop Effect and make your data very hard to interpret.
Pick colors that intuitive for your readers, but don't be rude or offensive.
If two of your items are more related than the others, consider giving them colors that are more similar to each other.
Some things in scientific illustrations have to be a certain color. Do your readers a favor and stick to what they expect.
For example, mitochondria are inexplicably always shown as red. They don't need to be firetruck red, but people expect them to be at least redish.